In Krebs-perfused lungs, endotoxin (E Coli LPS, serotype 0127:B8) injected directly into the perfusion system at a concentration of 5 |ig/mL did not affect PAP (n=8).
However, in lungs perfused with homologous blood, LPS induced a marked increase in PAP within 7 to 10 mins after administration. PAP reached a maximal, more than fourfold, increase usually between 60 and 80 mins. Such an increase was accompanied by symptoms of pulmonary edema. BAY X1005 10 |ig/mL, administered into the perfusion circuit 20 mins before LPS, lowered the rise in PAP induced by LPS by 72±2.1% (n=15), as measured 70 mins after LPS administration (Figure 2). Pulmonary protection by BAY was concentration dependent, and at a lower concentration of 3 |iM this compound inhibited the LPS-induced increase of PAP by 64±5% (n=15) (Figure 2).
Figure 2 Concentration-dependent inhibition of lipopolysaccha-ride- (LPS) induced (5 ^ig/mL) increase in pulmonary arterial pressure (PP) by Bay X1005 (BAY) compared with control experiments (LPS) in rabbit lung in situ reperfused with homologous blood. Bay X1005 (circles, 3 mM, n=8 and squares, 10 mM, n=8) or saline (control experiments; triangles, n=14) was administered into lung reperfusion cicuit 20 mins before LPS. Each point represents mean ± SD of n experiments . Efficient drugs that work for you exactly as expected may not be easy to find, but they are worth looking for. With this my canadian pharmacy you no longer need to look and wonder because you can purchase best quality drugs any moment.