The putative transmembrane and cytoplasmic regions of the mink LH receptor cDNA were cloned and sequenced. This portion of the receptor gene had sequence homology of 84%, 85%, 90%, and 91% with the murine, rat, porcine, and human LH receptor gene sequences and 64-65% with the rat, human, ovine, and bovine FSH receptor cDNA. Of 22 potential phosphorylation sites (serine, threonine, tyrosine residues) in the intracellular region of rat LH receptor, 19 were conserved in the deduced amino acid sequence of the mink LH receptor. Cheap Diskus Advair
The number and location of potential phosphorylation sites in the first and second intracellular loops were the same in the mink and rat. However, in the third intracellular loop, one of the three potential phosphorylation sites in the rat was shifted from a threonine to a methionine in the mink. Hipkin et al. reported that serine residues at amino acid positions 635, 639, 649, and 652 of the LH/ CG receptor were phosphorylated in cells treated with hCG and phorbol 12-myristate-13-acetate. In the mink, however, only two of these serine residues were conserved (639 and 652). A further six potential phosphorylation sites were present in the cytoplasmic tail of the mink LH receptor. Segaloff and Ascoli reported that cysteine residues are conserved among the species studied and that 11 of these are found in the transmembrane and cytoplasmic regions of the receptor. All 11 of these cysteine residues were conserved in the deduced amino acid sequence of the mink LH receptor, including two cysteine residues at amino acid positions 621 and 622 of the rat LH receptor, which are believed to be palmitoylated.