Local Release of Steroid Hormones: RESULTS(2)

RESULTS(2)

TNFa alone did not affect basal production of P4, A, or E2, but it suppressed the LH-stimulated release of A and E2 (p < 0.05; Fig. 2). IL-1p inhibited the release of A but stimulated the release of E2 (p < 0.05) without having any distinct effect on the release of P4 (Fig. 3). buy levaquin online

Effects of ET-1, TNFa, and IL-1/3 on the Release of PGE2

ET-1, TNFa, and IL-1p stimulated PGE2 release for a prolonged period (p < 0.05; Fig. 4). The infusion of ET-1 or TNFa after LH exposure induced a further increase of PGE2 release as compared with LH infusion alone (p <0.05; Fig. 4).

Effects of LH and Cytokines on the Release of ET-1

LH clearly stimulated ET-1 release, and the effect continued during the period of the experiment (p < 0.05; Fig. 5). The infusion of TNF-a or IL-1p after LH exposure further increased the ET-1 release as compared with LH infusion alone (p < 0.05). However, TNFa or IL-1p alone did not have any noticeable effect on ET-1 release.

FIG. 4. Effects of LH (5 ^g/ml), ET-1 (250 ng/ml), TNFa (100 ng/ml), and IL-1 p (10 ng/ml) on PGE2 release from microdialyzed bovine mature follicles in vitro. Data are expressed as a percentage of the basal release (100% = 24.6 ± 4.2 pg/ml; n = 6, mean ± SEM). *p < 0.05 vs. baseline; a vs. b and c vs. d: p < 0.05 during the same time period.


FIG. 5. Effects of LH (5 ^g/ml), TNFa (100 ng/ml), and IL-1 p (10 ng/ml) on ET-1 release from microdialyzed bovine mature follicles in vitro. Data are expressed as a percentage of the basal release of ET-1 (100% = 6.2 ± 1.3 pg/ml, n = 6; mean ± SEM). *p < 0.05 vs. baseline; a vs. b, and c vs. d: p < 0.05 during the same time period.

TABLE 1.

E A P pge2 ET-1
n (ng/ml) (ng/ml) (ng/ml) (pg/ml) (pg/ml)
Pre-LH surge 18 324 ± 21 28.5 ± 5.3 34.6 ± 5.3 355 ± 43 55.6 ± 5.9
Post-LH surge 6 296 ± 45 14.4 ± 3.1* 58.6 ± 5.6* 6145 ± 1 552** 65.0 ± 5.8

* p < 0.05.
**p < 0.01 vs. pre-LH surge group.

This entry was posted in Steroid Hormones and tagged Bovine Mature Follicles, Endothelin, Luteinizing Hormone, Prostaglandin, Steroid Hormones.