Local Release of Steroid Hormones: RESULTS(1)

Effects of LH on the Secretory Function of Mature Follicles

Infusion of LH clearly increased the release of P4, A, E2, and PGE2 (201 ± 15% for P4, 163 ± 9% for A, 186 ± 11% for E2, and 212 ± 12% of baseline for PGE2; mean ± SEM; p < 0.001). The increase in A release was observed only during the LH infusion, while the release of P4 remained at higher levels until 2 h after LH infusion. LH infusion induced increases in the release of both E2 (p < 0.05; Figs. 1-3) and PGE2 (p < 0.05; Fig. 4), and these stimulatory effects were maintained until the end of the experiment. The release of ET-1 was also increased after LH exposure (165 ± 23% of baseline; mean ± SEM; p < 0.05) and remained at high levels until the end of the experiment (p < 0.05; Fig. 5). buy ampicillin

Effect of ET-1, TNFa, and IL-1/3 on the Release of Steroid Hormones

ET-1 inhibited the release of A and P4, but it stimulated the release of E2 (p < 0.05; Fig. 1). The decrease in the release of A was observed only during the ET-1 infusion. However, the effect on P4 and E2 remained for a prolonged period.
Fig1Local Release
FIG. 1. Effects of LH (5 ^g/ml) and ET-1 (250 ng/ml) on P4, A, and E2 release from microdialyzed bovine mature follicles in vitro. Data are expressed as a percentage of the basal release of each hormone (100% = 14.1 ± 4.5 pg/ml for P4, 49.5 ± 10.5 pg/ml for A, and 25.2 ± 6.3 pg/ml for E2, respectively, n = 6; mean ± SEM). *p < 0.05 vs. baseline.

Fig2Local Release
FIG. 2. Effects of LH (5 ^g/ml) and TNFa (100 ng/ml) on P4, A, and E2 release from microdialyzed bovine mature follicles in vitro. Data are expressed as a percentage of the basal release of each hormone (n = 6; mean ± SEM). The baseline (100%) of each hormone was 15.1 ± 3.5 pg/ml for P4, 55.7 ± 12.5 pg/ml for A, and 20.2 ± 5.3 pg/ml for E2. *p < 0.05 vs. baseline; a vs. b and c vs. d: p < 0.05 during the same time period.

Fig3Local Release
FIG. 3. Effects of LH (5 ^g/ml) and IL-1 p (10 ng/ml) on P4, A, and E2 release from microdialyzed bovine mature follicles in vitro. Data are expressed as a percentage of the basal release of each hormone (n = 6; mean ± SEM). The baseline (100%) was 14.1 ± 4.5 pg/ml for P4, 59.5 ± 10.5 pg/ml for A, and 25.2 ± 6.3 pg/ml for E2. *p < 0.05 vs. baseline.

This entry was posted in Steroid Hormones and tagged Bovine Mature Follicles, Endothelin, Luteinizing Hormone, Prostaglandin, Steroid Hormones.