Erythromycin Inhibits Neutrophil Chemotaxis in Bronchoalveoli of Diffuse Panbronchiolitis: BAL Fluid

Additionally, there were no significant correlations between the recovery rate and NCA either in initial BAL fluid samples (r=0.017, p>0.6) or in repeated samples (r= —0.316, p>0.6). Albumin concentration and NCA did not correlate in either the initial or the repeated samples of BAL fluid (initial samples: r=0.102, p>0.6; repeated samples: r=0.254, p>0.6). Thus, we regarded NCA as being unaffected by recovery rate or by albumin concentration, and we, therefore, expressed without correction. Although BAL fluid samples from DPB patients may contain more bacterial endotoxin than samples from healthy subjects, we believe it to be that the endotoxin would not have affected the chemotaxis findings, both in the light of report by Issekutz and Bhimji that endotoxin did not induce neutrophil chemotaxis in the in vitro assay and in the light of our observation here that neither of the two endotoxin preparations (P aeruginosa serotype 10 and Salmonella typhosa; Sigma) tested induced neutrophil chemotaxis in the in vitro assay at concentrations 10 to 100 times higher than those inducing leukocyte infiltration in vivo (data not shown) Here.

Correlation Between Reduction in Neutrophil Percentage and Neutrophil Chemotactic Activity in Pre- and Post-EM Treatment BAL Fluid
Neutrophil chemotatic activity and neutrophil percentage in the BAL fluid of the 13 patients with DPB were compared before and after EM therapy. There was a significant correlation between the reduction in neutrophil percentage and NCA (r=0.737, p<0.01; Fig 2). Analysis of pre-EM treatment BAL fluid obtained from one patient with DPB (case 4) resulted in four peaks of activity; these were estimated to be 15,000, 8,000, 1,500, and 300 daltons (Fig 3). All four peaks of chemotactic activity in the BAL fluid from this patient were reduced after EM treatment (Fig 4).

Figure-2

Figure 2. Correlation between reduction in neutrophil chemotactic activity and neutrophil percentage in BAL fluid. The vertical and horizontal axes express the percent reduction of NCA and neutrophil percentage, respectively. There was a significant correlation between the reduction in neutrophil percentage and chemotactic activity (p<0.01).

Figure-3

Figure 3. Elution profile of concentrated BAL fluid in a patient with DPB (case 4) before EM treatment. Protein is shown in the upper panel (OD 280 nm) and the elution positions of proteins with known molecular weights are given. Chemotactic activity in the column fractions, shown on the ordinate as a percentage of the chemotactic response to 10-7 mol/L N-formyl-methionyl-leucyl-phenylalanine, is shown in the lower panel. Concentrated BAL fluid has four peaks of chemotactic activity.

Figure-4

Figure 4. Elution profile of concentrated post-EM treatment BAL fluid of the patient shown in Figure 3. Protein is located in the upper panel (OD 280 nm) and the elution positions of proteins with known molecular weights are given. Chemotactic activity in the column fractions, shown on the ordinate as a percentage of the chemotactic response to 10~7 mol/L N-formyl-methionyl-leucyl-phenylalanine, is shown in the lower panel. All chemotactic activities were reduced after EM treatment.

This entry was posted in Panbronchiolitis and tagged bronchoalveolar lavage fluid, diffuse panbronchiolitis, erythromycin, neutrophil chemotactic factor.