RESULTS Data acquisition and analysis were controlled by pCLAMP (Axon Instruments) and ASCD (Droogmans, Laboratorium voor Fysiologie, KU Leuven, Belgium).
Statistical analysis: Quantitative results are given as arithmetic means ± SEM of n experiments. P<0.05 was considered statistically significant (Student’s t test, paired observations).Effects on action potential: At a stimulation rate of 1 Hz, GE 70 concentration-dependently (1 |j,mol/L: n=6; 3 |j,mol/L: n=6; 10 |j,mol/L: n=6) and significantly (P<0.01) decreased V max without significantly changing membrane resting potential, action potential amplitude, and times to 20%, 50% and 90% repolarization. A new steady state V max was reached within 80 mins. The reduction of Vmax was use dependent, in that V max of the first action potential after a quiescent period of 110 mins in the presence of 10 |J.mol/L GE 70 (n=4) was identical to values recorded during the control period. On resumption of stimulation, V max quickly declined to a new steady state within 7.0±1.1 s (n=6) at 1 Hz. During pauses in stimulation, V max recovered from the use-dependent block caused by 10 |J.mol/L GE 70 at a time constant of 6.6±0.6 s (n=8). Reduction of Vmax by 10 |J.mol/L GE 70 (n=5) was frequency dependent (0.1, 1, 2, 3 Hz).
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