Apparently, P12 is synthesized by the translation at a constant level of P12 mRNA in the epithelium of mucosa folds, and the successive secretion from the epithelial cell results in its accumulation in the lumen. The positive correlation of P12 and its RNA message with the development of the seminal vesicle prompted us to assess the effect of androgen on P12 expression in the accessory sexual gland. Figure 5 displays the Western blot analyses of P12 in the tissue homogenates of seminal vesicles from mice (14 wk) treated in several ways. The protein disappeared in the tissue of mice that had been castrated 3 wk earlier (Fig. 5, lane 2). The protein was not detectable in the tissues of castrated mice that had received a daily injection of corn oil only (Fig. 5, lane 3). buy cheap antibiotics
The protein reappeared in the tissues of castrated mice after they had received daily injections of testosterone propionate in corn oil for 8 consecutive days (s.c., 5 mg/kg of body weight per day; Fig. 5, lane 4). As compared with the P12 level in seminal vesicles of normal adults (Fig. 5, lane 1), the absence of P12 in the control castrated mice and its recovery soon after the hormonal administration revealed androgenic stimulation of P12 expression in the seminal vesicle.
FIG. 5. Androgen dependence of P12 synthesis in seminal vesicle of adult mice. Western blot analysis of protein extract from mouse seminal vesicles of normal adults (lane 1), adults castrated 3 wk previously (lane 2) and receiving corn oil for 8 consecutive days (lane 3), and adults castrated 3 wk previously and receiving testosterone propionate in corn oil for 8 consecutive days (lane 4). Total protein extract (10 ^g) was used for each experiment. Detection of P12 protein is described in Figure 2 legend.