Abundant P12 appeared in the concretion of the glandular alveolar in the prostate and in the fluid in the lumen of both the seminal vesicle and coagulating gland. In the seminal vesicle, P12 was immunolocalized on the luminal epithelium of primary and secondary mucosa folds, but it was absent in smooth muscle cells, indicative of the secretion of P12 from the epithelium of mucosa folds to lumen. The secretion of P12 from the epithelium of mucosa folds was also noted in the coagulating gland. In the prostate, the secretion of P12 from the fold projecting into the lumen of glandular alveolus was obvious. birth control yasmin
Developmental Profile of P12 Expression in the Seminal Vesicle
It is well known that accumulation of SVS, which contains a group of proteins that constitute the major protein components of seminal plasma, becomes prominent in the postpubertal period. We compared the levels of P12 and its message in the seminal vesicle at different ages of male mice in order to assess whether the P12 expression coincided with the development of this accessory sexual gland. The P12 RNA message was undetectable in the mice younger than 3 wk old, increased remarkably when they were 4 wk old, and remained at that level thereafter (Fig. 4A). The protein level corresponding to this change was examined by Western blot analysis, shown in Figure 4B. The protein did not appear in mice younger than 4 wk old, but it increased progressively with age after 5 wk.
FIG. 4. Synthesis of P12 and its mRNA in mouse seminal vesicle at different ages. A) Western blot analysis for P12 in the tissue protein extract (30 ^g); B) Northern blot analysis for P12 mRNA in the total RNA (40 ^g), at ages shown. p-Actin mRNA of B was used as an internal control.