The diameter of the largest follicle increased as puberty approached, probably as a result of increased numbers of LH pulses. The number of LH pulses increased between Days 0 and 2 after follicular aspiration at 5, 4, 3, 2, and 1 mo prepuberty, and this increase could have resulted in the increased rate of growth of the largest follicle compared with that at 8, 7, and 6 mo prepuberty, when the number of LH pulses did not increase between Days 0 and 2 after follicular aspiration. The number of LH pulses increased between Days 6 and 8 after follicular aspiration at 8, 7, 6, 5, and 4 mo prepuberty, but the increase in diameter of the largest follicle was of lesser magnitude than that of the largest follicle at 3, 2, and 1 mo prepuberty. The increase in the number of follicles a 5 mm 9 days after follicular aspiration indicates that a new wave of ovarian follicular growth was beginning and that the largest follicle was in the early portion of the atresia phase and therefore unresponsive to the increase in the number of LH pulses. flovent inhaler
The number of LH pulses in postpubertal cattle was greater during the growth phase of the first waves of ovarian follicular development of the estrous cycle than during plateau and regression phases of development. In the present study, the number of LH pulses was greater during the selection phase of development of the largest follicle than during the growth phase each month prepuberty.