The stage of the wave of ovarian follicular development did have a significant influence on secretion of LH, FSH, and estradiol. This indicates the intricate communication between the ovarian follicle and the hypothalamic-pituitary axis. Because the extent and type of communication to the hypothalamic-pituitary axis, via estradiol and other ovarian factors, change with stage of the wave of ovarian follicular development, evaluation of endocrine and physiological variables related to reproduction without consideration of the stage of the wave of ovarian follicular development limits the value of data obtained. Buy Advair Diskus Online
Of all the interactions evaluated between day after follicular aspiration and month prepuberty, the only interactions observed were for frequency and amplitude of LH pulses and size of the largest follicle. This interaction indicated there were changes in the pattern of release of LH pulses that enhanced development of the largest follicle after follicular aspiration as time progressed from 8 to 1 mo prepuberty. Previous research from our laboratory indicates that the increase in frequency of LH pulses during the per-ipubertal period is a primary regulator of onset of puberty. The dramatic changes in the pattern of LH pulses (both frequency and amplitude) after follicular aspiration over 8 mo prepuberty serve to indicate the importance of change in the pattern of release of LH pulses in processes of sexual maturation of female cattle. This also further emphasizes the role of the secretory pattern of LH in stimulating development of the largest ovarian follicle in cattle.