Data from the present experiment indicate that the rate of growth of the largest follicle, and the number and amplitude of LH pulses change during waves of ovarian follicular development as the peripubertal period progresses. The rate of growth of the largest ovarian follicle was greater during the selection phase of follicular development, when the number of LH pulses was greater and concentrations of estradiol were lower, than during the growth phase of follicular development. As the largest follicle grew and developed during the early growth phase of follicular development peripuberty, concentrations of estradiol increased and the number of LH pulses decreased, resulting in a decreased rate of growth of the largest follicle. During the latter portion of the growth phase, the diameter of the largest follicle increased slightly, concentrations of estradiol decreased, and the number of LH pulses increased. ampicillin antibiotic
There was no interaction between day after follicular aspiration and month prepuberty for numbers of follicles < 5 or a 5 mm or for concentrations of LH, FSH, or estradiol. This indicates that the pattern of change in these variables over the 9 days subsequent to follicular aspiration was similar each month during the 8-mo prepubertal period. Most important, however, are the changes in these variables during waves of ovarian follicular development in prepubertal cattle.