Category Archives: Human Placenta

Expression of the Erythropoietin Receptor: MATERIALS AND METHODS(2)


Cell Isolation and Culture Procedures

TF-1, HeLa, and Jar cells were obtained from American Type Culture Collection (Rockville, MD). The erythroleu-kemic, bone marrow-derived TF-1 cells served as a positive control for EPO-R expression. These nonadherent cells were seeded in 75-cm2 vented tissue culture flasks and grown in RPMI-1640 medium with 1-5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; Pepro Tech, Rocky Hill, NJ), 10% fetal bovine serum (FBS), l-glutamine, and penicillin/streptomycin. buy cheap antibiotics

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Expression of the Erythropoietin Receptor: MATERIALS AND METHODS(1)

Human Placentas

Human placentas were obtained from women with normal pregnancies at term immediately after cesarean section for breech presentation or repeat cesarean section. Placentas were also collected from nulliparous women with the diagnosis of preeclampsia immediately after cesarean section (in the absence of any labor). Preeclampsia was defined according to standard criteria: onset of hypertension during late pregnancy with systolic and diastolic blood pressure > 140/90 on at least two occasions and urinary protein > 2+ on dipstick or > 0.3 g/24 h. First- and second-trimester placentas were obtained after elective terminations by vacuum evacuation and dilation and curettage, respectively. A total of 20 placentas were investigated. Collection of placentas was approved by the Institutional Internal Review Board of the Magee-Womens Hospital. birth control yasmin

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Expression of the Erythropoietin Receptor by Trophoblast Cells in the Human Placenta

Human Placenta

Erythropoietin is a 30.4-kDa glycoprotein critical to the survival, proliferation, and differentiation of erythroid precursor cells. The major sites of erythropoietin (EPO) production are the kidney and liver. However, other sites of EPO expression have been reported, including spleen and lung, bone marrow macrophages, early colony-forming cells, umbilical cord monocytes differentiated into a macrophage phenotype in vitro, and brain astrocytes. Most recently, our laboratory has reported that trophoblast cells of the human placenta express EPO, and we have speculated that, analogous to the effects of other colony-stimulating factors produced by and acting upon trophoblast cells, locally derived EPO is involved in placental development. Of course, fundamental to this hypothesis would be the demonstration of EPO receptor expression by trophoblast cells. buy flovent inhaler

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