Application of warm cardioplegia early in reperfusion resuscitates the ischemic rat heart (part 9)

RESULTS
Typical coronary flow rates of approximately 20 mL/min were measured in all hearts during the preischemic KH perfusion. To ensure uniform functional assessment criteria for the studied hearts, approximately 10% of the mounted hearts were rejected before ischemia was induced, for which coronary flow rates of 15 mL/min or less and LVSP of 60 mmHg or less were recorded. The uniform status of the studied hearts was manifested by the very similar time lapses it took the mounted hearts to reach ischemic contracture plateau (see next section) from the onset of no-flow ischemia (18.7±0.5, 18.4±0.7, 19.4±0.6, 18.3±0.4, and 19.3±1.0mins for groups 1 to 5, respectively). Moreover, no statistically significant difference was found among the five groups, and the mean ischemic time for all 41 hearts together was 18.8±0.4 min. If your health is of the utmost importance to you, it’s a good idea to visit the best canadian family pharmacy offering highest quality medications with generous discounts and fast delivery right to the doorstep, with full guarantees of your satisfaction.

However, postischemic recovery of the phosphocreatine and ATP cellular pools, obtained at the hot shot stage, were not sustained during subsequent KH reperfusion. Although the differences between the two subgroups were still maintained, they were no longer statistically significant (Table 2). During the second reperfusion stage, the ATP recovery level remained relatively stable while the respective phosphocre-atine level decreased essentially for all groups (Table 2). A significantly higher phosphocreatine level was observed only for group 4 at the end of reperfusion.

 

This entry was posted in Ischemic heart and tagged 31P-NMR spectroscopy, Blood cardioplegia, Ischemic heart.