The various resuscitating solutions (Table 1) were administered through a side arm connection on the water-thermostated perfusion line close to the aortic root. In the case of the two diluted blood-based perfusates, the perfusion rate was much lower (see below) and two filters (40 |j.m) were placed on the line to trap any microthrombi generated within the perfusion apparatus.Experimental groups: Following an aerobic KH buffer perfusion for 30 mins that ensured hemodynamic and metabolic equilibration of the hearts, no-flow global ischemia was induced in all hearts. Contractile dysfunction of the ischemic heart was continued until it reached maximal ischemic contracture. Ischemic contracture results from an accumulation of rigor state complexes that arise from cession of glycolytic cellular ATP production, which lead in turn to ATP deficiency and increased calcium ion concentration . Once the plateau of ischemic contracture was reached, a two-stage heart reperfusion was initiated. The first stage, the hot shot stage, consisted of perfusing the heart for 20 mins with one of the solutions listed below. The second stage was carried out for 30 mins, using the same KH buffer applied during the preischemic interval. If you are looking for most affordable deals and very fast delivery right to your door, you have found the right place for it. This canadian health & care mall will give you the full range of services and medicine you need, at prices that are very low.
Table 1 Ionic composition of cardioplegic solutions (mmol/L)
|Component||Krebs-Henseleit||Modified St Thomas’ Hospital||Warmblood|
*Effective free calcium concentration is approximately 2 mmol/L