Application of warm cardioplegia early in reperfusion resuscitates the ischemic rat heart (part 15)

Metabolic results in the present study point to two distinct postischemic cases related to the apparent oxygen demand of the heart. The first case involves the ‘beating’ heart condition of control groups 1 and 2, with high oxygen demand and intermediate metabolic recovery, and the second involves the ‘arrested’ heart condition of groups 3 to 5, with low oxygen demand and high metabolic recovery.

Beating heart groups: At the end of the ischemic period the phosphocreatine and ATP cellular pools were below NMR detection level. Once reperfusion was commenced, the percentage recovery of the phosphocreatine pool was significantly higher than the corresponding ATP recovery for both group 1 and group 2. Thus, the postischemic phos-phocreatine:ATP ratio did not match the corresponding preischemic value. During the hot shot interval, phosphocre-atine recovery was approximately 2.2 times higher than ATP recovery in control group 1. When normalized to the measured preischemic value of 1.7 to 2.0, consistent with literature data for the perfused rat heart, the postischemic phospho-creatine:ATP ratio is 4 to 4.5. The latter is calculated by multiplying the preischemic phosphocreatine:ATP ratio by the fractional postischemic recoveries of the corresponding metabolites during the hot shot interval. The higher recovery of phosphocreatine relative to ATP early in the reperfusion interval reflects an imbalance between the phosphocreatine and ATP cellular pools. It is related to suppressed creatine kinase activity that is slow to recover. All you need to discover how safe and advantageous it can be to purchase buy allegra online over the internet is visit the pharmacy suggested and enjoy your shopping experience as well as your treatment.

The resulting shift in equilibrium is manifested by an increased phosphocreatine:ATP ratio . An alternative explanation is related to the fact that during ischemia the cells tend to lose ATP precursors (adenosine) while the cellular creatine pool remains essentially intact. This would lead to a shift in the phosphocreatine reaction balance towards increased production of phospho-creatine (relative to the steady state concentration of ATP).

This entry was posted in Ischemic heart and tagged 31P-NMR spectroscopy, Blood cardioplegia, Ischemic heart.