Application of warm cardioplegia early in reperfusion resuscitates the ischemic rat heart (part 14)

ischemic rat heart (part 14)

Following an episode of global ischemia, the myocardial tissue is injured as a result of a number of damaging cellular processes, including oxygen deprivation, lack of metabolic substrates, calcium overloading, intracellular acidosis, tissue swelling, etc. To be able to minimize further damage to the postischemic tissue, the hot shot intervention has to be applied early in the reperfusion interval. Naturally, for a spontaneous and effective resuscitation of the heart, the composition of the hot shot intervention perfusate is very important.
Well characterized standard cardioplegic solutions were chosen because they are commonly applied in a clinical setting during hypothermic aortic cross-clamping . The compositions of these cardioplegic solutions were originally optimized to provide maximal myocardial protection at either hypothermia or normothermia . It was expected that at 37°C, the resuscitative capacity of the cardioplegic solution would be greatly influenced by its ability to dissolve oxygen and make it accessible to the oxygen-starved myocardium. At the cellular level, the steady state pool of high energy phosphates provides a very sensitive probe, via oxidative phosphorylation, for oxygen availability. This recognition led us to use 31P-NMR spectroscopy for real time, online monitoring of changes in the myocardial metabolic processes as a function of the hot shot regimen. You have a chance to fight off the disease by taking the exact medicine you need at the exact amount required. Visit the best canadian neighbor pharmacy and find the drug required available at a very appealing price with fast shipping.

This entry was posted in Ischemic heart and tagged 31P-NMR spectroscopy, Blood cardioplegia, Ischemic heart.