Application of warm cardioplegia early in reperfusion resuscitates the ischemic rat heart (part 12)

Intracellular pH: During the preischemic period, the recorded mean pH of the tissue was initially 7.06±0.07 (n=41), decreasing to 6.11±0.11 after 10 to 12 mins of global ischemia. Intracellular Pi peak, indicating an acidic pH, still noticeable in spectra taken early in the hot shot period, completely disappeared by the end of the hot shot stage of reperfusion. An exception was observed for the group 1 hearts that maintained residual ‘acidic’ Pi signal even in spectra taken at the end of the reperfusion period (Figure 1, bottom trace). These spectra displayed two Pi peaks, corresponding to acidic and normal pH values of6.10±0.15 and 6.97±0.09, respectively.In hearts treated with cardioplegic hot shot and in group 2, the residual acidic Pi resonance disappeared from the spectrum as early as the end of the hot shot stage (data not shown). Hemodynamic recovery: As indicated from the metabolic results discussed above, the respective recovery of LVDP and +dP/dt parameters for groups 3 to 5 were significantly better than those recorded for groups 1 and 2 (Table 3). You will always be able to buy the required amount of medicine you need at this wonderful and fully licensed pharmacy that can take good care of you, no matter if you need purchase alegra at large amounts or just a bit of it to finish your treatment.

Table 3 Preischemic hemodynamic parameters of perfused rat hearts and their percentage recovery values (± SEM) at the end of 50 mins of postischemic perfusion

Group LVDP(mmHg) % + dP/dt (mmHg/s) % CF(mL/min) % LVDPxHR(mmHg/min) %
1 (n = 10) 70±3.0 56±7.5 1500±105 52±6 10.8±0.5 62±4 20000±1000 45±8
2 (n=7) 87±8.5 60±5 2000±210 56±5.5 11.9±0.5 45±5.5 26000±3000 47±7
3 (n=10) 83±7 88±10* 1800±125 78±7.5* 11.3±0.5 59±4 24000±2000 64±8*
4 (n=7) 75±4 86±6.5* 1500±105 87±7.5* 12.1±1 65±4 24000±1500 68±5.5*
5 (n=7) 74±3.5 77±6.5* 1500±60 74±5* 10.5±0.5 66±5 22000±1500 66±5.5*

*Significant at P<0.05 compared with group 1; fSignificant at P<0.05 compared with group 2. Group 1: Krebs-Henseleit (KH) buffer (control); group 2: warm blood diluted with normal KH buffer (control); group 3: warm blood diluted with modified St Thomas’ cardioplegic solution; group 4: high potassium KH cardioplegic solution; group 5: modified St Thomas’ cardioplegic solution. CF Coronary flow; dP/dt Pressure-time derivative; HR Heart rate; LVDP Left ventricular developed pressure

This entry was posted in Ischemic heart and tagged 31P-NMR spectroscopy, Blood cardioplegia, Ischemic heart.