Enzyme activity was determined as described by Par-ia et al. in the mitochondrial fraction from the rate of NADH oxidation at 340 nm. The reaction mixture consisted of 1 ml of 100 mM potassium phosphate buffer, pH 7.5, containing 50 mM oxaloacetate and 20 mM NADH. The rate of NADH oxidation following the addition of 10 ^l of follicular pellet fraction was recorded as described in the previous section. MDH activity was expressed as millimoles NADH oxidized per minute per milligrams protein. Buy Advair Diskus Online
There were enough samples from each group to run each enzyme assay in duplicate. An average of the two replicates represented the mean enzyme activity for each pooled sample (n = 1) for each ovary. All assays were done separately using homogenates of classes 1 and 2 preantral follicles obtained from 3 premenopausal women (n = 3), and values representing 3 ovaries were used to calculate the mean ± SEM. The results were analyzed by one-way ANOVA with Scheffe’s test, and Student’s t-test whenever necessary. The level of significance was at 5%.